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HPV-siRNA对Hela细胞生长抑制作用的实验研究 被引量：1

Growth Inhibitive Effect of Hela by HPV-siRNA
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摘要 以HPV18 E6基因为靶位,研究siRNA对宫颈癌Hela细胞生长的抑制作用。设计并合成siRNA,脂质体siNEO FX转染Hela细胞,分别用MTT法,双层软琼脂克隆形成试验和流式细胞术分析了siRNA对Hela细胞体外生长增殖活力、细胞周期分布的作用。siRNA作用后的Hela细胞增殖速度减慢,软琼脂克隆形成率降低,G0/G1期细胞比率增加。HPV18 E6 siRNA能抑制Hela细胞体外生长增殖能力并诱导细胞周期重新分布。 Inhibitive effect on growth of cervical cancer cell line Hela by small interfering RNA （siRNA） targeting HPV18E6 was studied. Specific siRNA was designed and synthesized, and transfected Hela with liposome siNEO FX, then analyse the role of siRNA on proliferation vitality outside the Hela cells, cells periodic distribution by MTT method, double-layer soft agar clone-forming assay, and flowing cell technology. The results showed that Hela cell proliferation speed decreased, clone formation on soft agar decreased, and cells on G0/G1 stage ratio increased, suggested that HPV18 E6 siRNA could inhibit Hela cell growth and capability of proliferation, and induce cell cycle to redistribute.
作者 王雪莲 尹香菊 王冬冬 安春丽
机构地区 中国医科大学基础医学院病原生物学教研室 中国医科大学第二临床学院妇产科
出处 《微生物学杂志》 CAS CSCD 2007年第3期30-34,共5页 Journal of Microbiology
基金 国家自然科学基金项目(30670916) 辽宁省教育厅资助项目(05L519)
关键词 SIRNA HPV HELA 抑制作用 small interfering RNA （siRNA） human papillomarovirus （HPV） Hela inhibitive effect
分类号 R373.13 [医药卫生—病原生物学]
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参考文献13

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同被引文献23

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微生物学杂志
2007年 第3期

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