Treatment of Sleep Deprivation-induced Circadian Rhythm Disorder by Applying Garlic Cream on Acupoint Shenque （CV 8）

目的：观察神阙穴贴敷大蒜膏对抗睡眠剥夺所致昼夜节律紊乱的整复作用。方法：将20名健康成年男性随机分为正常对照组（A）、睡眠剥夺组（B）、睡眠剥夺并贴药组（C），对B和C组同时进行48小时睡眠剥夺，A组不做任何处理，观察A组及睡眠剥夺后B组和C组的血清单胺类神经递质（去甲肾上腺素和5-羟色胺）的浓度变化。结果：三组的去甲肾上腺素（NE）均呈典型的昼夜节律性（A和C组P〈0．01，B组P〈0．05）。A组峰值为158．377，出现在10：56；B组峰值291．529，出现在19：44左右；C组峰值255．964，出现在17：06左右。B组与A组相比峰相位出现了明显的偏移，C组的峰相位较B组有所恢复。A组的5-羟色胺（5-HT）有典型的近似昼夜节律性（P〈0．01），B组和C组的节律消失（P〉0．05）。A组峰值为196．563，大约出现在13：10。结论：大蒜膏穴位贴敷可以调整人体紊乱的昼夜节律，在一定程度上促进紊乱的节律的恢复，是一种简便有效的抗昼夜节律紊乱的方法。

Objective： To observe the regulative effect of applying garlic cream on acupoint Shenque （CV8） on circadian rhythm disorder induced by sleep deprivation. Methods： Twenty healthy adult men were randomly divided into normal group （group A）, sleep deprivation group （group B） and treatment group （group C）. Subjects in group B and C received 48-hour sleep deprivation, and in the meantime subjects in group C were treated by applying garlic cream on acupoint Shenque （CV8）, while subjects in group A received no any treatment, then contents of serum noradrenaline （NA） and 5-hydroxytryptamine （5-HT） were detected. Results： The contents of NA in three groups all appeared typical circadian rhythm （P〈0.01 when group A is compared with group C, and P〈0.05 when group A is compared with group B）. The peak value in group A was 158.377 and appeared at 10：56, peak value in group B was 291.529 and appeared at 19：44, peak value in group C was 255.964 and appeared at 17：06. The peak phase in group B shifted more obviously when compared with group A, and the peak phase in group C recovered slightly when compared with group B. The contents of 5-HT in group A showed typical circadian rhythm （P〈0.01） and the circadian rhythms in group B and C disappeared （P〉0.05）, the peak value in group Awas 196.563 and appeared at about13： 10. Conclusion： The application of garlic cream on acupoint Shenque （CV8） can adjust the disturbed circadian rhythm and accelerate the recovery of circadian rhythm. It is a simple and effective therapeutic method for adjusting circadian rhythm disorder.