适用于光学显微镜的细胞培养芯片

Cell Culture Biochips for Optical Microscopy

虽然细胞分子生物学近年来发展很快,但是细胞培养的方法变化很慢。将近一个世纪以前发明的有盖培养皿至今仍在常规使用,但它已经不适合许多用来观察和分析活体细胞内发生的变化的现代光学分析方法。这篇文章描述了一种细胞培养芯片装置,它能有效地用于细胞培养和随后的高端光学分析,比如荧光和共焦显微镜方法。

Despite recent rapid changes in cellular molecular biology, methods of cell culture have been slow to change. Petri dishes, introduced nearly a century ago, and still routinely employed, are not suitable containers for many of the modem optical methods that are used to investigate and analyze the processes occurring inside living cells. This article describes a cell culture slide system that is efficient for cell culture and the subsequent high-end optical analysis of cells by, for example, fluorescence or confocal microscopy.