HIV/AIDS患者血清IL-18浓度变化与辅助受体CCR5/CXCR4表达的关系研究

Correlation between the expression of CCR5/CXCR4 and raised serum levels of interleukin-18 in HIV carriers/AIDS patients

目的研究人类免疫缺陷病毒/获得性免疫缺陷综合征(HIV/AIDS)患者血清白细胞介质(IL)-18浓度的变化,及其与病毒载量、CD4+T细胞表面辅助受体CCR5/CXCR4表达的相关性。方法应用酶联免疫吸附试验(ELISA)对42例HIV/AIDS患者及12例正常对照者的血清IL-18浓度进行定量检测,分析其变化,并对其变化与病毒载量的相关性进行分析。应用流式细胞仪检测全部HIV/AIDS患者CD4+T细胞表面辅助受体CCR5/CXCR4的表达情况,并分析其与病毒载量及血清IL-18浓度变化之间的相关性。结果42例HIV/AIDS患者血清平均IL-18浓度显著高于正常对照组(P<0.01),且按美国疾病控制与预防中心(CDC)1993年标准,CDC C期显著高于A期(P<0.01)。所有患者血清IL-18浓度变化与HIV病毒载量之间呈正相关(P<0.05),与辅助受体CX-CR4的表达也呈正相关(P<0.01)。HIV病毒载量与辅助受体CXCR4的表达具有正相关性(P<0.05)。结论HIV/AIDS患者体内持续增长的IL-18加剧了其免疫功能的紊乱和低下,IL-18可能与疾病本身的致病机制有关。

Objective To investigate the serum IL-18 levels in HIV carriers/AIDS patients and their relations with viral load and the expression of coreceptors CCR5/CXCR4 on CD4^＋ T lymphocytes. Methods Serum IL-18 levels of 42 HIV carrlers/AIDS patients and 12 healthy controls were determined by ELISA, and their relations with serum viral load were analyzed. Flow-cytometry was used to detect the coreceptors CCR5/CXCR4 expressions on CD4^＋ T lymphocytes in whole blood samples from 42 HIV carriers/AIDS patients. The correlation of the coreceptors expression with serum IL-18 levels and viral load was analyzed. Results Forty two HIV carriers/AIDS patients had significantly raised IL-18 levels compared with healthy controls （P 〈 0.01）, and there was significantly higher serum IL-18 levels in AIDS group C than AIDS in AIDS group A according to 1993 CDC standard （USA） （P 〈 0.01 ）. A significantly positive correlation among serum IL-18 levels, viral load and the CXCR4 expression on CD4^＋ T lymphocytes was identified （ P 〈 0.05 ）. Conclusions The persistently raised serum IL-18 levels in HIV carriers/AIDS patients will possibly exacerbate their immunodeficiency status, and may also be involved in the pathogenesis of the disorder itself.