两种四环素类抗生素厌氧生物毒性试验研究

Study on Toxicity to Anaerobes of Two Kinds of Tetracycline Antibiotics

在35℃中温厌氧条件下,采用间歇试验法,通过测定甲烷累积产量,研究脱水土霉素和强力霉素两种四环素类物质的厌氧生物降解性,利用相对活性来判断两类物质对产甲烷菌的抑制程度。试验结果表明:脱水土霉素浓度低于200 mg/L时产生轻度抑制,高于200 mg/L产生中度抑制;强力霉素低于200 mg/L时产生中度抑制,高于200 mg/L时对产甲烷菌产生重度抑制,厌氧污泥活性很难恢复。

A batch anaerobic experiment was conducted at 35 ℃ to determine anaerobic biodegradability of two kinds of tetracycline antibiotics, i. e. , doxycyeline and anhydrooxytetracycline through measuring accumulative methane production. The concept of relative activity was adopted to determine the inhibition on methanogenic bacteria. The result showed that when the concentration of anhydrooxytetracycline increased from lower level to higher than 200 mg/L, the inhibition would increase from slight level to medium level. While for the doxycycline, when the concentration increased from lower level to higher than 200 mg/L, the inhibition increased from medium level to strong level. In this case, the activity of anaerobic sludge was difficult to resume.