实时荧光定量PCR检测乳腺癌SOCS2基因表达

SOCS2 gene expression in breast carcinoma by real-time polymerase chain reaction

目的检测乳腺良恶性组织中细胞因子信号转导抑制因子(supressors of cytokine signaling,SOCS2)的 mRNA表达及其临床病理意义。方法以18S rRNA为内对照采用实时荧光定量PCR法,定量测定新鲜乳腺癌组织、癌旁组织和乳腺良性增生组织中,SOCS2 mRNA的表达并分析其与临床病理指标的关系。结果各组阳性率:乳腺癌35.00%(14/40)、癌旁乳腺组织100%(15/15)和乳腺良性增生组织81.25%(13/16),其表达之间的差异具有统计学意义(P<0.05);乳腺癌组织的SOCS2表达水平与TNM分期和淋巴结转移呈明显的相关性(P<0.05)。结论SOCS2在乳腺癌中的表达可能提示患者具有较好的预后。

Purpose To investigate the significance of SOCS2 mRNA expression in the carcinogensis and development of breast carcinoma, Methods Real-time quantitative reverse transcription polymerase chain reaction（RQ-RT-PCR） technique was applied for detecting SOCS2 and 18SrRNA mRNA expression in the tissues of breast carcinoma, adjacent tissues and breast benign lesions. The relationship to clinical and biological parameters was also discussed, Results Positive expression ratio of the SOCS2 mRNA in breast carcinoma, their adjacent tissues and breast benign lesion was 35, 00% （ 14/40）, 100.00% （15/15） and 81.25% （ 13/16）, respectively, The expression of SOCS2 mRNA of breast carcinoma was lower than that of non-cancerous tissue （P 〈 0. 05）. SOCS-2 expression was inversely related to TNM classification and lymph node metastasis （P 〈 0. 05）. Conclusions The expression of SOCS2 gene in the breast carcinoma probably implies a better prognosis.