应用组织芯片研究p63及其同系物在肺癌中的表达

p63 isoforms in lung neoplasms: Tissue microarray and immunohistochemical studies

目的研究p63蛋白及其同系物TAp63、△Np63在肺癌组织中的表达,并初步探讨其生物学意义。方法选取183例肺癌、30例肺良性病变的石蜡标本,应用组织芯片技术和免疫组织化学方法观察三种蛋白在肺良、恶性病变中的表达情况。结果p63蛋白及其同系物TAp63、△Np63三者在肺鳞状细胞癌、腺癌、细支气管肺泡癌、小细胞癌的表达具有一致性(r>0.75,P<0.01),且在鳞状细胞癌中的表达率均高于其它类型(P<0.01);在鳞状细胞癌中△Np63表达率(74.6%)略高于TAp63(67.2%),但无统计学意义(P>0.05)。三种蛋白的表达与患者性别、年龄、肿瘤分化程度、肿瘤大小、有无淋巴结转移及临床分期不相关(P>0.05)。p63、TAp63及△Np63在肺癌旁组织或肺良性病变的肺泡上皮细胞和支气管纤毛柱状上皮不表达,而只表达于支气管上皮的基底细胞和支气管腺的肌上皮细胞。结论p63基因在不同类型的肺癌中作用不同,在鳞状细胞癌的发生发展中起着重要的作用。两种同系物TAp63和△Np63在鳞状细胞癌中也呈高表达,其机制有待研究。p63可作为肺鳞状细胞癌与其它类型癌鉴别的重要依据。

Purpose To detect the expression of p63 isoforms （TAp63, A Np63） in 4 types of lung neoplasms and to gain insight their biological significance in carcinogenesis of lung neoplasm. Methods One hundred and eighty-three formalin-fixed, paraffin-embedded eases of lung neoplasms and 30 matched surroundings specimens as control were examined for p63, TAp63 and A Np63 protein expression by imunohistoehemistry using tissue mieroarray. Results The expression of the three protein in squamous cell carcinomas （ SCC）, adenoeareinomas （AC）, bronehioloalveolar carcinoma （BAC） and small cell lung carcinomas （SCLC） were closely correlated with each other （ r 〉 0. 75 ,P 〈 0. 01 ） and all significantly higher in SCC than the other types （ P 〈 0. 01 ）. The matched surrounding tissues only showed nuclear p63 immunoreaetivity in basal cells and myoepithelium of bronchi mueosal glands. The expression was not correlated with elinieopathologieal characteristics. Conclusions p63 plays a great role in the carcinogenesis of lung SCC, and TAp63/△Np63 may both display certain function. These results may also help distinguish SCC from other three types of lung neoplasms in diagnostic pathology.