非小细胞肺癌survivin、p53和COX-2蛋白的表达

Expression of survivin, p53 and COX-2 in non-small cell lung cancer

目的探讨非小细胞肺癌组织中survivin、p53和环氧合酶-2(COX-2)蛋白表达及其在肺癌发生、分化程度、淋巴结转移以及预后评估中的意义。方法采用SP法免疫组化技术检测70例非小细胞肺癌组织中的survivin、p53和COX-2表达情况。结果survivin和COX-2阳性表达与肺非小细胞癌组织的分化程度、有无淋巴结转移及临床TNM分期有关(P<0.01);p53阳性表达与非小细胞肺癌组织的分化程度有关(P<0.01)。同时发现,survivin、p53和COX-2(■～■)阳性表达率明显高于(+～■),差异有显著性(P<0.01)。这3种抗体中在非小细胞肺癌中的阳性表达占76%。结论survivin、p53和COX-2的表达在非小细胞肺癌组织的浸润、转移和发展中具有重要意义,有助于对非小细胞肺癌的诊断及预后的评价。

Purpose To investigate the expression of survivin, p53 and COX-2 in non-small cell lung cancer （NSCLC） and their clinical significance. Methods The expression of survivin, p53 and COX-2 in 70 cases of NSCLC were determined by immunohistochemistry with SP method. Results The expression of survivin and COX-2 was related to histopathological types, lymphatic metastasis and TNM grade of lung cancer （P 〈 0. 01 ）, while that of p53 was correlated with histopathological types of lung cancer, and the positive rates of over-expression （＋＋＋～＋＋＋＋） were much more than that of low-moderate （ ＋～＋＋）. There was significant difference between two groups （P 〈 0.01 ）. The positive rate of COX-2 expression was the highest among three proteins in NSCLC . The COX-2 positive ratios was 76%. Conclusions Our results indicate that the expression of survivin, p53 and COX-2 proteins may play an important role in invasion, metastasis and development in non-small cell lung cancer and it helps to diagnose lung cancer and to evaluate the prognosis.