喉鳞状细胞癌转化生长因子β_1与热休克蛋白70表达的相关性研究

Expression and significance of TGF-β_1 and HSP70 in human laryngeal squamous cell carcinoma

目的:探讨转化生长因子β1(TGF-β1)与热休克蛋白70(HSP70)在喉鳞状细胞癌的表达情况及意义。方法:采用免疫组织化学SP法结合计算机图像分析系统对53例喉鳞状细胞癌和48例癌旁组织中TGF-β1和HSP70的表达进行检测。结果:喉鳞状细胞癌组织中TGF-β1和HSP70均有不同程度的表达;喉鳞状细胞癌TGF-β1的表达明显低于癌旁组织(P<0.05);与之相反,喉鳞状细胞癌HSP70的表达明显高于癌旁组织(P<0.05)。喉鳞状细胞癌组织中TGF-β1和HSP70的表达均与肿瘤组织分化程度密切相关(P<0.01或P<0.01),且两者的表达水平呈明显负相关(r=-0.87,P<0.01)。结论:TGF-β1和HSP70的阳性表达与喉鳞状细胞癌组织学分级密切相关,它们共同参与了喉鳞状细胞癌的发生、发展及恶性进程。

Objective：To investigate the expression of TGF-~I and HSP70 in human laryngeal squamous cell carcinoma. Method：The expression levels of TGF-β1and HSP70 in 53 specimens of human laryngeal squamous cell carcinoma and 48 specimens of para-carcinoma were detected by immuno- histochemistry and computer assisted image analysis. Result：The expression of TGF-β1 was decreased in carcinoma tissues compared with para-carcinoma tissues （ P〈0.05）, while the expression of HSP70 was increased （ P〈0.05）. Both the expression of TGF-β1 and HSP70 were significantly correlated with the differentiation of laryngeal squamous cell carcinoma （ P〈0, 01 orP 〈0.05）. However, there was a negative correlation between TGF-β1 and HSP70 （ r =-0.87, P 〈0.01）. Conclusion： TGF-β1 and HSP70 play an important role in malignant behaviors of human laryngeal carcinoma.