凋亡相关蛋白Fas及caspase-3在膀胱移行细胞癌中表达及临床意义

Expression of apoptosis-related protein Fas and caspase-3 in bladder transitional cell carcinoma and their clinical significance

目的:探讨凋亡相关蛋白Fas及caspase-3在膀胱移行细胞癌中的表达及其临床意义。方法:应用SP免疫组织化学法检测45例膀胱移行细胞癌及10例正常膀胱黏膜组织石蜡切片中Fas和caspase-3表达的情况,并结合临床资料进行分析。结果:Fas在膀胱移行细胞癌标本中的表达阳性率为46.7%(21/45),而在正常对照组中阳性表达率为100%;caspase-3在膀胱移行细胞癌标本中的表达阳性率为37.8%(17/45),与对照组阳性率为90%(9/10)相比差异有统计学意义(P<0.05)。Fas的表达与膀胱移行细胞癌的组织学分级,初发和复发及肿瘤数目之间差异有统计学意义(P<0.05),但与临床病理分期无关;Caspase-3的表达与膀胱移行细胞癌的初发和复发相关(P<0.05),但与组织学分级,肿瘤数目,临床分期均无关。相关性分析表明,膀胱移行细胞癌中Fas的表达与caspase-3表达呈正相关。结论:Fas在膀胱癌组织中选择性表达降低且与膀胱移行细胞癌的分化程度密切相关的,caspase-3蛋白在膀胱移行细胞癌中表达下降,因此Fas及caspase-3蛋白对于判断膀胱移行细胞癌预后有重要临床指导意义。

Objective：To investigate the expression of apoptosis-related proteins Fas and caspase-3 in bladder transitional cell carcinoma （BTCC） and their clinical significance. Methods： The bladder tissues were isolated from 45 BTCC patients and 10 healthy controls and embedded in paraffin. The expression of Fas and caspase-3 were detected by streptavidin peroxidase immunohistochemical method. The results were analyzed associated with clinical records. Results： The positive rate of Fas expression was 46.7 % （ 21/45 ） in TCC and 100% in the 10 normal bladder tissues. The positive rate of caspase-3 expression was 37.8 % （17/45） in BTCC and 90% （9/10） in normal control group. The difference was significant （P 〈0.05 ）. The expression of Fas was significantly related with the histological grades, the initiation of BTCC, either primary or recurrent, and the tumor number and but not associated with clinicopathological stages. While the expression of caspase-3 correlated with the initiation of BTCC, either primary or recurrent ; but it did not correlate with the histological grades, tumor numbers, and the pathological stages of BTCC. There was a positive correlation between the expression of Fas and caspase-3 in BTCC. Conclusion： The decrease in the selective expression of Fas is closely associated with the differentiation degree of BTCC. The expression of caspase-3 is down-regulated in BTCC. Expression of Fas and caspase-3 are important to indicate the prognosis of BTCC.