催化信号放大法的改进

Modification of catalyzed signal amplification method

目的：探讨酪胺与过氧化物酶发生酶促反应后，用热缓冲液洗以进一步提高和改善催化信号放大法（CSA）的敏感性和特异性。方法：应用7种不同类型的一抗，2张不同肿瘤的组织芯片，同时进行EnVision法、LsAB法、标准CSA法和改进CSA法染色，比较敏感性和特异性。结果：改进CSA法最为敏感，其次是标准CSA法、EnVision法和LsAB法，改进CSA法的敏感性较标准CSA法高2～3倍。4种方法背景染色基本一致，细胞定位准确。结论：改进后的CSA法的稳定性、敏感性好于标准CSA法。

Objective：To verify whether hot PBS washing after the enzymatic reaction between tyramine and horseradish peroxidase can improve the sensitivity and specificity of catalyzed signal amplification （CSA）. Methods： Using 7 different types of primary antibodies and 2 tumor microarray system, we carried out EnVision, LSAB, Standard CSA, and modified CSA staining and compared their sensitivities and specificities. Results： The modified CSA method was the most sensitive one among the 4 methods, followed by standard CSA, EnVision, and LsAB method. The sensitivity of modified CSA method was 2-3 times higher than that of standard CSA. The 4 methods had similar background staining and had exact localization of cells. Conclusion： The modified CSA is more stable and sensitive than traditional CSA staining method.