摘要
目的:探讨降钙素基因相关肽(CGRP)和神经生长因子(NGF)对脑组织缺血再灌注的保护作用及机制。方法:采用颈动脉负压分流法制作大鼠脑缺血再灌注模型,采用TUNEL法、免疫组织化学SABC法及显微图像分析检测海马及皮质内神经元凋亡和核因子κB(NF-κB)的表达。结果:大鼠缺血再灌注海马及顶叶皮质内神经元凋亡数增加而NF-κB免疫阳性反应产物较正常组减少,注射CGRP或NGF后神经元凋亡数减少,NF-κB免疫阳性反应产物明显高于缺血再灌注组,两者联合应用效果更加显著。结论:CGRP和NGF抑制缺血神经元凋亡,参与NF-κB的调节,两者对缺血神经元有协同修复作用。
Objective: To investigate the expression of nuclear factor κB (NF-κB) in the hippocampus after cerebral ischemia and reperfusion in rats, and explore the protective effects and mechanism of calcitonin gene-related peptidc (CGRP) and nerve growth factor (NGF) on the brain tissue. Methods: A novel model of cerebral ischemia and reperfusion was induced by carotid artery negative pressure shunt in rats; the expressions of NF-κB and apoptosis cell in the hippocampus and cortex were detected with SABC immunohistoehemical and TUNEL method, and analyzed by microimage system. Results: The expressions of NF-κB and apoptosis cell in the hippocampus and cortex were decreased after cerebral ischemia and reperfusion; compared with this, the expressions of NF-κB reaction positive cells in CGRP or NGF group were increased; and the effect of the combined use of CGRP and NGF was better. Conclusion: Our results indicate that CGRP and NGF depress cell apoptosis and participate in regulating the expression of NF-κB in ischemic neurons, and they may cooperate with each other.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2007年第3期320-322,350,共4页
Chinese Journal of Anatomy
基金
辽宁省教育厅科学研究计划(05L442)