降钙素基因相关肽和神经生长因子对脑缺血再灌注大鼠海马及顶叶皮质神经元凋亡及核因子-κB表达的调节

Effects of CGRP and NGF on NF-κB expression and neuron apoptosis in the hippocampus and parietal cortex during cerebral ischemia and reperfusion in rats

目的:探讨降钙素基因相关肽(CGRP)和神经生长因子(NGF)对脑组织缺血再灌注的保护作用及机制。方法:采用颈动脉负压分流法制作大鼠脑缺血再灌注模型,采用TUNEL法、免疫组织化学SABC法及显微图像分析检测海马及皮质内神经元凋亡和核因子κB(NF-κB)的表达。结果:大鼠缺血再灌注海马及顶叶皮质内神经元凋亡数增加而NF-κB免疫阳性反应产物较正常组减少,注射CGRP或NGF后神经元凋亡数减少,NF-κB免疫阳性反应产物明显高于缺血再灌注组,两者联合应用效果更加显著。结论:CGRP和NGF抑制缺血神经元凋亡,参与NF-κB的调节,两者对缺血神经元有协同修复作用。

Objective： To investigate the expression of nuclear factor κB （NF-κB） in the hippocampus after cerebral ischemia and reperfusion in rats, and explore the protective effects and mechanism of calcitonin gene-related peptidc （CGRP） and nerve growth factor （NGF） on the brain tissue. Methods： A novel model of cerebral ischemia and reperfusion was induced by carotid artery negative pressure shunt in rats; the expressions of NF-κB and apoptosis cell in the hippocampus and cortex were detected with SABC immunohistoehemical and TUNEL method, and analyzed by microimage system. Results： The expressions of NF-κB and apoptosis cell in the hippocampus and cortex were decreased after cerebral ischemia and reperfusion; compared with this, the expressions of NF-κB reaction positive cells in CGRP or NGF group were increased; and the effect of the combined use of CGRP and NGF was better. Conclusion： Our results indicate that CGRP and NGF depress cell apoptosis and participate in regulating the expression of NF-κB in ischemic neurons, and they may cooperate with each other.