温下法与温涩法对溃疡性结肠炎患者IL-8、TNF-α的影响比较

Comparison on influences of warm-purgation therapy and warm-astringing therapy on contents of IL-8 and TNF-α in patients with ulcerative colitis

目的探讨温下法与温涩法在溃疡性结肠炎(UC)免疫炎性损伤方面的不同作用机制。方法采用双抗体夹心ABC-ELISA法检测治疗前后温下组、温涩组和对照组血清白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)的含量,采用二步免疫组化法检测治疗前后3组结肠组织IL-8、TNF-α的表达水平,比较3组治疗前后血清和结肠组织IL-8、TNF-α的变化。结果温下法与温涩法均降低血清和结肠TNF-α水平,温下法在降低血清和结肠IL-8方面疗效优于温涩法。结论温下法的作用机制可能是通过抑制TNF-α与IL-8的表达,阻抑以中性粒细胞为主的炎性细胞聚集,减少急性炎症反应;温涩法的作用机制可能是通过抑制淋巴细胞产生TNF-α,来减轻慢性炎症损伤。

Objective To investigate the different mechanisms of warm -purgation therapy and warm- astringing therapy in immunologic injury of ulcerative colitis. Methods Before and after the treatment the serum contents of interleukin-8 （IL-8） and tumor necrotic factor-α （TNF-α） were detected by ABC- ELISA method in the warm-purgation group, warm-astringing group and control group, the expressions of IL-8 and TNF-α in colonic tissue were examined by two-step immunohistochemistry method in three groups and the changes of IL-8 and TNF-α in serum and colonic tissue were compared in three groups. Results Both warm-purgation therapy and warm-astringing therapy co serum and colonic tissue. The warm-purgation therapy had a better effect uld induce the TNF-α level in to decrease IL-8 level in serum and colonic tissue than warm-astringing therapy. Conclusion The effective mechanism of warm-purgation therapy may be related to that it can inhibit the expressions of IL-8 and TNF-α, prevent inflammatory cells （mostly neutrophil ） from aggregation and relieve acute inflammatory reaction. The effective mechanism of warm-astringing therapy may be related to that it can prevent lymphocyte from producing TNF-α to relieve chronic inflammatory injury.