前列通瘀汤对前列腺蛋白所致慢性非细菌性前列腺炎大鼠前列腺组织细胞因子影响的研究

Influence of Qianlietongyu Decoction on cytokines of prostatic tissue in rats with chronic nonbacterial prostatitis induced by prostatic protein

目的探讨前列通瘀汤对前列腺蛋白所致慢性非细菌性前列腺炎(CPPS)大鼠治疗作用机理。方法采用腹腔注射百白破疫苗,多点皮下注射大鼠前列腺蛋白提纯液和福氏完全佐剂的混悬液免疫方法制造大鼠CPPS模型。大鼠分为正常组、模型组、塞来昔布组和前列通瘀汤大、中、小剂量组。30d后处死大鼠,取前列腺组织,ELISA法检测前列腺组织匀浆白细胞介素-1β(IL-1β)、环氧化酶-2(COX-2)、前列腺素E2(PGE2)、β-内啡肽(β-EP)的含量。结果前列通瘀汤大剂量组与塞来昔布组均可显著降低大鼠前列腺组织中IL-1β、COX-2、PGE2的含量,升高β-EP的含量。前列通瘀汤大剂量组与塞来昔布组比较无显著差异(P(0.05)。结论前列通瘀汤对CPPS的治疗作用与前列通瘀汤能调节前列腺组织炎性和细胞因子含量密切相关。

Objective To investigate the therapeutic mechanism of Qianlietongyu Decoction in rats with chronic nonbacterial prostatitis induced by prostatic protein. Methods The rat model of chronic nonbacterial prostatitis was established by intraperitoneal injection of DPT vaccine, multiple intradermal injections of the suspension of prostatic protein purification with Freund＇ s completed adjuvant. Then the rats were divided into the normal group, model group, celecoxib group and different Qianlietongyu Decoction groups （ in prostatic tissue was low, middle or high taken. In prostatic cyclooxygenase-2 （ COX-2 ）, prostaglandin does respectively）. After 30 days the rats were killed and homogenate the contents of interleukin-1β （IL-1β）, E2 （PG E2） and β-endorphin （β-EP） were detected by enzyme linked immunosorbent assay （ELISA）. Results In the Qianlietongyu Decoction group in high dose and celecoxib group the contents of IL-1β, COX-2 and PG E2 were decreased significantly and β- EP content was increased. The comparison between these two groups was not different significantly （ P 〉 0.05）. Conclusion The therapeutic effect of Qianlietongyu Decoction is closely related to that it can inhibit prostatitis and regulate cytokine contents.