摘要
利用PEG-高Ca2+高pH法对烟草属的普通烟草K326和Nicotiana rustica、N.alata、N.plumbaginifolia、N.debneyi分别进行种间叶肉原生质体融合试验,结果表明基因型对烟草叶肉原生质体融合有明显影响。通过试验还对影响烟草叶肉原生质体融合及其成株的其它相关因素进行了探讨。
In this paper tobacco interspecific protoplast fusion was studied with mesophyU protoplasm isolated from K326 and N.rustica,N. alata,N.plumbaginifolia,N.debneyi respecdvely by PEG-high Ca^2＋/pH. The obtained results showed that genotype affected tobacco protoplast fusion obviously. Some other factors affecting protoplast fusion and plant regeneradon were also studied here.
出处
《热带农业科技》
2007年第2期29-32,共4页
Tropical Agricultural Science & Technology
关键词
烟草
原生质体融合
影响因素
tabacco
protoplast fusion
plant regeneration
influencing factor
