摘要
目的:研究血管内皮生长因子受体(VEGFR3)在舌癌淋巴转移中的作用,探索舌癌淋巴转移的机制。方法:采用免疫组化和RT-PCR技术,对90例不同分期的舌癌肿瘤进行VEGFR3的检测,与10例正常舌黏膜标本进行对照。采用SAS6.12软件进行单因素方差分析。结果:通过与正常组对照,Ⅰ、Ⅱ、Ⅲ期肿瘤中VEGFR3的表达无显著性差异(P>0.05),Ⅳ期肿瘤中VEGFR3的表达较正常对照组显著升高并具有显著性差异(P<0.05)。肿瘤各组间比较无显著性差异。转移组与非转移组之间也无显著性差异。结论:VEGFR3参与微淋巴管的生成,随着肿瘤的临床分期增高,其表达虽呈缓慢增高趋势,但尚不足以作为判定是否发生淋巴转移的客观指标。
Objective： To investigate the expression of VEGFR3 during lymphatic vessel formation and its value in lymphatic metastasis of tongue cancer. Methods： 90 cases of tongue cancer in different stages were chosen for this the study. All the cases were examined immunohistochemically with streptavidinbiotin method and reverse transcription-polymerase chain reaction （RT-PCR） to determine the expression of VEGFR3. SAS 6.12 soft ware was used for one-way analysis of variance. Results： The expression of VEGFR3 in cancer stage IV was significant higher than normal tissue（P〈 0.05）. There was no significance of difference between tumor group, metastasis group and nonmetastasis group. Conclusion： The expression of VEGFR3 was increased smoothly with the changes from the tumor stage I to IV .These results suggest that VEGFR3 would have the tendency to promote lymphatic metastasis, but VEGFR3 alone was not enough to judge whether lymphatic metastasis has occurred.
出处
《口腔颌面外科杂志》
CAS
2007年第2期123-125,129,共4页
Journal of Oral and Maxillofacial Surgery
关键词
舌癌
VEGFR3
淋巴转移
tongue cancer
VEGFR3
lymphatic metastasis
