β-C在急性出血坏死性胰腺炎中对GSH及MDA的影响

Influence of β-carotene on GSH and MDA in acute hemorrhagic necrosis of pancreas

目的:探讨β-胡萝卜素(β-C)在急性出血坏死性胰腺炎(ANP)中对GSH及MDA影响。方法:Wistar大鼠随机分为3组:A组,ANP组;B组,β-C处理组;C组,假手术组,测定各组谷胱甘肽(GSH)、丙二醛(MDA)的变化。结果:同一时点比较发现:A组胰腺组织中GSH明显下降(P<0.05)、MDA明显升高(P<0.05);B组则GSH下降幅度较小,与A组比较差异有显著性(P<0.05),MDA升高幅度小,与A组比较差异有显著性(P<0.05)。结论:ANP时胰腺组织中内源性抗氧化物质GSH显著下降,MDA增加,β-胡萝卜素可作为氧自由基清除剂明显减轻ANP大鼠胰腺细胞损伤。

Objective; To explore the influence of β-carotene on glutathione （GSH） and malonyl dialdehyde （MDA） in acute hemorrhagic necrosis of pancreas （AHNP）. Method： Wistar rats were randomly divided into three groups. AHNP group ;β-carotene group;control group. After AHNP rat models were made by using the Aho method, the levels of MDA and GSH were measured. Results： The GSH level in the AHNP group was remarkably decreased in acute hemorrhagic necrosis of pancreas （P 〈 0. 05） ,while MDA was significantly increased （P 〈 0.05） . The GSH level in the β-carotene group was higher than that in the AHNP group （P 〈 0. 05） . The MDA level was lower than that in the AHNP group （P 〈 0.05） . And the histopathologic damage was attenuated to a certain extent. Endogenous anti- oxide GSH decreases remarkably in the pancreas in acute hemorrhagic necrosis of pancreas, MDA increases. Conclusion： β-carotene as ROS elimination agent reduce evidently the damage of rat pancreas cells.