EGCG对顺铂诱导Hela细胞凋亡的影响及其相关机制

Effect of EGCG on apoptosis of cervical cancer Hela cells induced by DDPand the related mechanism of tumor cell apoptosis

目的:研究EGCG单独或协同顺铂对人宫颈癌Hela细胞体外生长的影响及其相关机制。方法:本实验采用MTT法检测药物对细胞生长的抑制作用;HE染色法观察细胞凋亡的形态学变化;TUNEL法检测细胞凋亡率;免疫组化法检测NF-κB和Cyclins D1表达。结果:50μg/mL浓度的EGCG作用12h的抑制率为12.6%。400μg/mL浓度的EGCG作用48h的抑制率上升到78.3%。12h,50μg/mL的EGCG诱导细胞的调亡指数为8.3%;0.1μg/mL的DDP诱导细胞的调亡指数为6.7%;而两者合用诱导细胞调亡指数为13.7%(P<0.01)。EGCG作用细胞后可显著抑制NF-κB和CyclinD1表达(P<0.05)。结论:EGCG能抑制Hela细胞的生长及诱导其调亡,并且对DDP有增敏作用。可能通过抑制NF-κB和CyclinD1表达而起作用。

Objective：To study the different effects of EGCG alone or its cooperation with DDP on in vitro growth of Hela cells of human cervical carcinoma. Methods ：In this experiment, MTT colorimetry was adopted to examine the inhibitory effect of drugs on cell growth, and the morphological changes of cell apoptosis were observed by HE method. The rate of cell apoptosis was detected with TUNEL method. The expression of albumen NF-κB and Cyclins D1 was investigated by immunohistochemical techniques. Results：The inhibitory ratio was 12.6%, after 50μg/mL of EGCG was added to in vitro growing Hela cell lines for 12 h. The inhibitory ratio was 78.3%,after 400μg/mL of EGCG was added to in vitro growing Hela cell lines for 48h （P 〈 0.05）. The apoptosis inhibition ratio was 13.7% （P （0.05） when the 50μg/ mL of EGCG was jointly used with 0. μg/mL DDP for 12h. The effect was stronger than when EGCG alone or DDP alone was used （P 〈 0.05） . EGCG could decrease the expression of NF-κB and Cyclins D1 （P 〈 0. 05） . Conclusion：EGCG can inhibit the Hela cell growth and induce the cell apoptosis in human cervical carcinoma. EGCG has an enhanced sensitivity effect on DDP, and EGCG could decrease the expression of NF-κB and Cyclins D1.