卷烟总粒相物及烟碱盐中尼古丁的HPLC含量测定

The Determination of Nicotine in Cigarette Grain Phase and Nicotine Salt

本文利用反相高效液相色谱法（ＲＰＨＰＬＣ）测定了卷烟总粒相物及烟碱盐中尼古丁的含量。色谱条件为：固定相Ｓｈｉｍ－ｐａｃｋＣＬＣ－Ｃ８（５μ，６．０×１５０），流动相０．１ｍｏｌ／ＬＨ３ＰＯ４－ＫＨ２ＰＯ４缓冲液（流速１．５ｍｌ／ｍｉｎ，ｐＨ１．９５），检测波长２６２ｎｍ，进样５μＬ），柱温４０℃。与传统的紫外分光光度法和气相色谱法相比，该法具有准确、简便。

In this paper, the content of nicotine in cigarette grain phase and nicotine salt are determined by Reversed Phase High Performance Liquid Chromatography (RPHPLC). The chromatogram conditions are: fixed phase shim-pack CLC-Cs (5μ 6.0×150), moving phase 0.1mol/L, H 3PO 4-KH 2PO 4 buffer (flow rate 1.5ml/min, PH 1.95),detection wavelength 262nm, sample injection 5μl, pillar temperature 40℃. Compared with the traditional ultraviolet spectrophotometry and gas chromatognaphy, this method is noted for its accuracy, convenience, high-speed and economical function.