中国明对虾乙酰基专一性凝集素分离纯化方法的研究

Study on methods of purification of acetyl group specificity lectin from shrimp Fenneropenaeus chinensis

为研究凝集素在中国明对虾天然免疫中的作用，利用3种不同物质——N-乙酰葡萄糖胺（GlcNAc）、胎球蛋白（Fetuin）和小牛黏液素（BSM）为配基，分别与3种柱材料结合进行亲和层析，从中国明对虾血淋巴中纯化凝集素。这3种亲和层析均得到了一种相同的凝集素FCL-1，经SDS-PAGE证明FCL-1的分子量为168kDa。利用新鲜小鼠血细胞进行分离纯化同样获得了这种凝集素。FCL-1与乙酰基糖类和唾液酸类糖蛋白均具有较强的结合特性。

A natural lectin from the serum of the shrimp Fenneropenaeus chinensis was purified by three different methods of affinity chromatography using N-acetylglucosamine-coupled agarose, Fetuin-coupled agarose and Bovine mucin （BSM）coupled Sephamse 4B. This lectin was named FCL-1 and its inactive form had a molecular mass estimate of 168 kDa. This kind of lectin was also purified oy using fresh red blood cells （RBC） from mouse. In HA-inhibition assays performed with several carbohydrates and glycopmteins FCL-1 showed a distinct specificity for acetyl group in carbohydrates and Siagroup in sialoglycoconjugates.