摘要
分别采用商品化的酶联免疫吸附试验(ELISA)抗体检测试剂盒和血凝抑制(HI)试验方法对152份临床血清样本和标准阴阳性血清进行新城疫病毒(NDV)抗体检测,比较间接 ELISA 和 HI 两种方法的相关性。研究结果表明,间接 ELISA 和 HI 两种方法呈显著相关,二者间的相关系数为0.9261。间接 ELISA 和 HI 两种方法检测 NDV 抗体的阳性符合率为96.8%,阴性符合率为92.9%,间接 ELISA 方法是一种敏感、特异、快速的血清学诊断方法,可以用于临床样本的大规模检测。
One hundred and fifty-two field sera and several standard sera were assayed for antibodies to Newcastle disease virus （NDV） by a commercial enzyme-linked immunosorbent assay （ELISA） kit and haemagglutination inhibition （HI） test respectively. The titers between the two tests showed a significant correlation between the two tests and the correlation coefficient was 0.9261. The two tests had a 96.8% coincident positive rate and a 92.9% coincident negative rate. Furthermore the ELISA is a sensitive,specific and rapid serologic method for ND diagnosis and is useful for the analysis of a large quantity of samples.
出处
《中国家禽》
北大核心
2007年第4期14-16,19,共4页
China Poultry
关键词
新城疫
间接ELISA
HI
相关性
Newcastle disease
indirect enzyme-linked immunosorbent assay
haemagglutination inhibition test
correlation
