超滤/DEAE离子交换层析法分离纯化IgY

Separation and Purification of IgY by Ultrafiltration and DEAE Ion-exchange Chromatogram

应用平板式膜超滤技术与 DEAE 离子交换层析法对卵黄免疫球蛋白(IgY)进行分离纯化。首先,卵黄液经过缓冲液稀释分离;然后,用平板式膜组件对其超滤,并且分析了各种因素对其的影响,确定分子截留量为100 ku,操作压力差为0.12 MPa、进料温度为30℃,pH5.2,截留液抗体回收率达到91.89%,纯度为86%。经 DEAE 离子交换层析得到高纯度的 IgY。

Yolk immunoglobulin （IgY）was separated and purified by plate-type membrane uhrafiheration and DEAE ion-exchange chromatogram, Firstly,the yolk liquor was pretreated by dilution and separation,and then IgY was separated by uhrafilteration using plate-type membrane module. Moreover,we studied many factors affecting uhrafilteration and optimal product was achieved at 0,12 MPa,30℃,pHS.2 with IOOku（MWCO）. In this condition,about 91.89% antibody was recovered,and its purity reached to 86%. Finally,the purity of antibody was enhanced by DEAE ion-exchange chromatogram.