陕西省鸡肾型传染性支气管炎病毒的分离鉴定

Isolation and Identification of Avian Nephropathogenic Infectious Bronchitis Virus from Shaanxi Province

从临床表现呼吸道传染病的20个发病鸡群中先后分离出18株病毒,通过鸡胚感染、血清学试验、分子生物学试验、动物回归试验证明其中13株是冠状病毒科的鸡传染性支气管炎病毒,3株为新城疫病毒,2株为H9禽流感病毒。该13株中的BJ2、FF2、YL2、B01、G5、YX、WH、WG分离毒具有很强的嗜肾性和致病力,这8个陕西流行株的血清型与W118毒株相同,而与IBVGray株、W93株有一定的差异。结果表明,陕西地区主要流行鸡肾型传染性支气管炎,且流行株具有强致病力。

Eighteen isolates were isolated successively from 20 suspected flocks with respiratory infection symptoms, and 13 of them were identified to be of infectous bronchitis virus（IBV）,which belonged a member of the family Coronaviridae,3 of the rest were Newcastle disease virus （NDV）,the last 2 were H9 subtype of avian influenza virus. By chicken embryo infection,serological test,molecular biological test and animal infection tests,the results of animal infection test showed that the 8 isolates,including BJ2,FF2,YL2,B01 ,GS,YX,WH and WG,had strongly nephropathogencity, moreover,the results of neutralization test in e＇mbryonated eggs revealed the control strain Wl18 could neutralize the 8 isolates completely,while Gray and W93 could not do that,they were able to neutralize W118 to a considerable high degree, In conclusion,the main prevalent strain of IBV in Shaanxi province was nephropathogenetic infectious bronchitis virus.