核因子-κB和环氧合酶-2在胃癌组织中的表达与肿瘤微血管形成的关系

Expression of NF-κB and COX-2 and the Relationship with Angiogenesis in Gastric Carcinoma

目的研究核因子-κB(NF-κB)和环氧合酶-2(COX-2)在胃癌组织中的表达及其与肿瘤微血管密度(MVD)之间的关系。方法采用免疫组织化学SP法检测142例胃癌中NF-κB、COX-2的表达和MVD,以相应的癌旁正常组织(30例)作对照。结果NF-κB在胃癌组织中的阳性表达率为62.0%(88/142),显著高于对照组(2/30,P<0.01);NF-κB的表达与胃癌淋巴结转移、组织学类型和远处转移等临床病理指标显著相关(P<0.05)。COX-2在胃癌组织中的阳性表达率为64.1%(91/142),在对照组无表达,二者比较差异有极显著性意义(P<0.01),且COX-2的表达与是否有淋巴结转移显著相关(P<0.01)。NF-κB和COX-2的表达呈正相关(r=0.380,P<0.01)。NF-κB阳性表达组或COX-2阳性表达组胃癌MVD显著高于其阴性表达组。结论NF-κB和COX-2在胃癌的发生发展中起着重要作用,促进了胃癌的血管生成。

Objective To explore the expression of NF-κB and COX-2 and the relationship with microvessel density （MVD） in human gastric carcinoma. Methods The expression of NF-κB, COX-2 and MVD in cancer tissue of 142 cases of gastric carcinoma were examined by immunohistochemical SP technique. The corresponding para-cancerous tissues in 30 cases served as control group. Results The positive expression rate of NF-κB was 62.0% in gastric carcinoma tissue, significantly higher than that in control group （P〈0.01）. The positive rate of NF-κB was closely related to local lymph node metastasis, histological differentiation grade and distant metastasis （P〈0.05）. The expression of COX-2 in gastric carcinoma （64.1%） was higher than that in control group （P〈0.01） and was closely related with local lymph node metastasis （P〈0.01）. NF-κB expression was positively correlated with COX-2 （r= 0. 380, P〈0.01）. The MVD in human gastric carcinoma positive for the NF-κB or COX-2 expression was obviously higher than that in human gastric carcinoma negative for the NF-κB or COX-2 expression. Conclusion NF-κB and COX-2 may play an essential role in the occurrence and development of gastric carcinoma. They can promote the angiogenesis of gastric carcinoma.