高效液相色谱法测定广西粉葛中葛根素的含量

Determination of Puerarinin Content in Guangxi Radix puerariae thomsonii by HPLC

目的 采用HPLC法测定广西粉葛中葛根素的含量。方法 色谱柱Phenom enex Gem in i C18柱（250 mm×4.6 mm,5μm）;流动相：乙腈（A）-0.4%磷酸水溶液（B）,采用梯度洗脱;流速：1.0 m l/m in;检测波长：250 nm;柱温：30℃。结果 葛根素在800-2400 ng范围内峰面积与进样量呈良好的线性关系。回归方程为A=7.682×10^4C＋5.885×10^4,r=0.999 3（n=5）。其平均回收率为99.49%,RSD为0.75%（n=5）。结论 该方法简便易行,结果准确,可作为广西粉葛药材质量控制的有效方法。

Objective To determine the content of puerarin from Guangxi Radix puerariae thomsonii by HPLC. Methods The isolation was conducted on a phenomenex gemini C18 column （250 rmm × 4.6 mm , 5 μm ） , the mobile phase consisted of acetonitnle （A） -0.4% phosphoric and （B） in a gradient model, the flow rate wes 1.0 ml/min, the detection wavelength was 250 nm and the column temporature was 30℃. Results The content of puerarin showed good linearity in the range of 800 ng -2400 ng,the regression equation was A =7. 682 × 10^4C ＋5. 885 × 10^4 ,r =0.999 3 （n =5）, and the average recovery was 99.49%, RSD was 0.75% （n = 5 ）. Conclusion This method is simple, rapid, accurate and reliable. It can be used for quality control of Guangxi Radix puerariae thomsonii.