摘要
目的优化野菊花总黄酮提取工艺。方法在分析单因素的基础上,以总黄酮得率为响应值,采用响应面实验设计方法(RSD)对其提取工艺进行研究。结果最佳工艺参数为乙醇浓度56.6%,提取温度80℃,液固比为19.6,提取时间为3.2 h,提取1次,该工艺条件可使总黄酮得率达4.64%。结论该工艺条件可为工业生产提供参考数据。响应面试验设计法可在连续范围内进行分析,优于现在普遍采用的只能分析离散条件的正交实验设计。
Objective To examine the procedure for the chemical extraction of the flavonoids in Chrysanthemum indicum L. Methods The procedure for the extraction was performed with the recovery yield of flavonoids as the response. Response surface deslgn（RSD） was applled to find the optimum parameters ,which is based on one-factor experiment. Results It was found that the procedure could achieve a good result when the sample was infiltrated with 19.6-fold 56.6% ethanol-water solution and mixed around at 80℃ for 3.2 h. The recovery yield of flavonoids got to 4.64% once extraction. Conclusion The procedure of the extraction can be helpful to the factory-scale production.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第3期648-650,共3页
Lishizhen Medicine and Materia Medica Research
关键词
响应面
野菊花
黄酮
Response surface
Chrysanthemum indicum L.
Flavonoids
