滨州市部分地面水体细菌耐药性分析

Antibiotic resistance of bacteria in ground water body of Binzhou

目的为了了解滨州市内地面水体中细菌的耐药性,并探讨其与耐药质粒谱之间的关系。方法采集滨州市内4个地面水体的水样,分别检测水样菌落形成单位(colony-forming unit,CFU)以及氨苄青霉素耐药率,用K-B法对氨苄青霉素耐药菌株分别做7种不同抗生素的药敏试验,然后提取这些菌株的质粒,用EcorI酶切质粒电泳后分析酶切质粒谱。结果样本氨苄青霉素耐药率达11.7%~49.8%。氨苄青霉素耐药菌株对其他3种不同的抗生素分别有12.5%~25%不等的耐药性,未找到耐西拉司丁钠的菌株。耐氨苄青霉素以及一种非β内酰胺类抗生素的菌株占32.5%,耐氨苄青霉素以及两种或者以上的非β内酰胺类抗生素的菌株占20%。酶切质粒谱共找到从815 bp^12.2 kb的63种大小不等的酶切片断。结论滨州市地面水体中细菌耐药性普遍存在,且多重耐药性明显;对细菌耐药性的监测是十分有意义而且有必要的。

Objective To survey the condition of antibiotic resistance in ground water body of Binzhou, and to study its relationship with plasmid profiles. Methods Water samples were collected from 4 places of ground water body of Binzhou, then CFU was counted and the antibiotic resistance rate was analyzed. K-B method was used to practice the susceptibility test with 5 antibiotics, then the antibiotic resistance spectrums were analyzed. Results The ampicillin resistance rate of the samples varies from 11.7% to 49. 8%. The ampicillin resistant isolates have different antibiotic resistance rate which varies from 12.5% to 25% relevant to 3 other antibiotics, and the cilastain resistance was not found . Isolates with the trait of ampicillin resistance and one non-β-lactam antibiotic reached 32. 5% , and isolates with the trait of resistant to ampcillin and two or more non-β-lactam antibiotics reached 20%. There are 63 plasmids bands which varys from 815bp to 12.2kb in plasmid profiles. Conclusion The antibiotic resistance in ground water body of Binzhou was prevalent, and the phenomenon of multidrug resistance is obvious too. It is significant and necessary to monitor the antibiotic resistance status.