川芎嗪对大鼠急性放射性肠炎小肠的作用

Effect of Ligustrazini on Rat Small Intestine in Acute Radiation Enteritis

目的:探讨川芎嗪对急性放射性肠炎大鼠小肠的营养作用。方法:22只SD大鼠,给予X线8Gy剂量的全腹部照射,建成急性放射性肠炎大鼠模型,分成模型对照组、川芎嗪治疗组(川芎嗪组)和正常对照组(正常组)。连续7d腹腔注射给药后,取相应部位的空、回肠,测定其蛋白质及DNA含量,在光镜下观察和图像分析仪器测定其相关的形态学指标。结果:模型对照组空、回肠出现粘膜坏死、脱落,而川芎嗪组粘膜完整,测值显示川芎嗪组空、回肠绒毛高度、隐窝深度、粘膜厚度和全层厚度以及蛋白质和DNA含量均显著大于模型对照组。结论:川芎嗪能加速急性放射性肠炎受损小肠粘膜的修复,对维持小肠粘膜结构和功能的稳定性有良好的作用。

Objective To study the effect of Ligustrazini on the morphology of rat small intestine in acute rediation enteritis Methods Twenty - two SD rats were randomly divided into three groups. To the control group（ n = 8） and ligustrazini group（ n = 8） whole abdominal radiation was performed, then physiologic saline and ligustrazini respectively were given peritoneal injection continuously for 7 days. The normal group（n = 6）received no irradiation. Other indices were measured by using histochemical analysis, light microscopy and image analysis system. Results The ligustrazini group after radiation had a significant increase in gut protein and DNA content, villus height, crypt depth, the thickness of mucosa and entire wall and number of metaphase mitoses per crypt. Conclusion Ligustrazini can accelerate the healing of the injured small intestinal epithelium, maintain small the normal structure and function of the small intestine.