克拉霉素的电化学反应机理研究与应用

Investigation on Electrochemical Reaction Mechanism of Clarithromycin and Its Application

应用线性扫描伏安法、循环伏安法、常规脉冲伏安法等电化学手段并结合紫外吸收光谱研究了药物克拉霉素(clarithromycin,CAM)在pH1.8～9.2 Britton-Robinson缓冲溶液和0.05mol·L-1NaOH溶液中的电化学行为.在所研究的pH范围,CAM分别产生P1,P2,P3,P4四个还原波,其中P1,P2,P4三个波均为其药效活性基团C-9位羰基的还原所产生.实验结果表明:在pH1.8～5.7的B-R缓冲溶液条件下所获得的P1波为两电子不可逆弱吸附还原波;在6.0<pH<9.2的B-R缓冲溶液中,CAM产生P2和P3两个波,其中P2为两电子不可逆还原波,P3为催化氢波.在0.05mol·L-1NaOH溶液中,CAM产生的P4波是一个单电子的不可逆吸附还原波.根据P4波的峰电流iP与CAM浓度的线性关系,建立了CAM含量测定的新方法.

The electrochemical behavior of clarithromycin （CAM） was studied in pH 1.8-9.2 Britton-Robinson buffer solution and 0.05 mol·L^-1 NaOH solution by means of linear sweep voltammetry, cyclic voltammetry, normal pulse voltammetry and UV spectrophotometry. Over the studied pH range, four reduction waves P1, P2, P3 and P4 of CAM were achieved, three of which were the reduction of C = O at C-9. CAM yielded one wave P1 as pH of B-R buffer solution was changed from 1.8 to 5.7. P1 was an irreversible and weak adsorption reduction wave involving two electrons. Additional two waves P2 and P3 were exhibited in pH 6.0-9.2 buffer solution. The former was an irreversible reduction wave involving two electrons, and the latter was a catalytic hydrogen wave. P4 was attained in 0.05 mol·L^-1 NaOH solution, which was an irreversible and adsorption reduction wave involving one electron. Based on the linear relation between the peak current of P4 and the concentration of CAM, a new method for determination of CAM was described.