13种石斛属植物遗传多样性的AFLP分析

ANALYSIS OF GENETIC DIVERSITY AMONG 13 CHINESE SPECIES OF DENDROBIUM BASED ON AFLP

采用AFLP技术对13种石斛属植物的遗传多样性进行了分析。选择性扩增引物组合E+ACT/M+CAC、E+AAC/M+CAC和E+ACA/M+CAC分别对这13种材料进行扩增,得到丰富的条带。在100-300bp共得到346条带,多态性带342条,多态性百分率为98.8%。聚类分析结果表明,在相似系数0.54处,可将13种材料分为Ⅰ、Ⅱ、Ⅲ类。Ⅰ类包括:串珠石斛、铁皮石斛、广东石斛、重唇石斛、晶帽石斛、细叶石斛、滇桂石斛、报春石斛、玫瑰石斛、球花石斛;Ⅱ类包括:鼓槌石斛;Ⅲ类包括:美花石斛(花,浅红)、美花石斛(花,淡白)。AFLP分析结果与传统分类学的结果基本一致,表明该标记技术对石斛属植物的遗传多样性和分类研究是可行的。

Amplified fragment length polymorphism （AFLP） was assessed in 13 Chinese species of Dendrobium. Three primer combinations E＋ACT/M＋CAC, E＋AAC/M＋CAC and E＋ACA/M＋CAC were used for selective amplification. Between 100bp and 300bp, a totle of 346 fragments were detected, in which 342 are polymorphic. The rate of polymorphism was 98.8%. The AFLP data were analyzed using Nei ＆ Li similarity coefficient and UPGMA Cluster method. The result indicated that the 13 species were clustered into three groups on coefficient level 0.54. Group Ⅰ included D. falconeri,D, officinale ,D. wilsonii, D. hercoglossura, D. crystallinum. D. hancockii, D. guangxiense, D. primulinum, D. crepidatum, D. thyrsiflorum. Group Ⅱ included D. chrysotoxum. Group Ⅲ included D. corresponding to the classification result by using the traditional method. sii. This outcome was It is concluded that AFLP markers can be used on the studies of genetic relationships and classification of Dendrobium.