3,5-二硝基水杨酸测定还原糖含量的条件探讨

Conditions for photometric determination of reducing sugar by using dinitrosalicylic acid reagent

对３，５－二硝基水杨酸测定还原糖含量的条件研究表明：较灵敏的比色波长在４８２～４８４ｎｍ之间；针对４８２～４８４ｎｍ，５２０ｎｍ，５４０ｎｍ等３个比色波长，其最佳待测浓度依次为５～７０μｇ／ｍＬ，５～１６０μｇ／ｍＬ，５～２００μｇ／ｍＬ；待测样品和标准样品的缓冲介质与ｐＨ值必须保持一致；显色时间为６ｍｉｎ。

Factors affecting the determination were studied.It is suggested that other than 520nm and 540 nm,482～484nm are more sensitive wavelengths for determination.The suitable concentration ranges are 5～70μm/ml,5～160μm/ml and 5～200μg/mL for 482nm～484nm,520 and 540 nm respectively.The buffer medium and pH value should be the same for the sample and the standard.The boiling time for developing should be about 6 min.The determination could be carried out about 2 h.