Interaction between antigen presenting cells and autoreactive T cells derived from BXSB mice with murine lupus

全身的豺狼座 erythematosus (SLE ) 是包含多重系统和机关的典型自体免疫病。宽大的证据建议反应 T 房间玩的那辆汽车在这自体免疫的混乱的发展的一个枢轴的角色。这研究被承担调查在介绍房间(APC ) 和反应 T 房间(ATL1 ) 克隆从豺狼座容易的 BXSB 老鼠获得了的一辆汽车的抗原之间的相互作用的机制。ATL1 房间，在 gamma 光线照耀前后的任何一个，能激活天真的 B 房间，由 B 细胞增殖试金决定了。巨噬细胞 fromBXSB 老鼠能刺激在一个应答者 / 激发器(R/S ) 放松 ATL1 房间的增长 1/2.5 的比率。树枝状的房间(DC ) 是为 aper 房间基础上的 ATL1 房间的更强大的激发器。刺激巨噬细胞的能力的 T 房间和 B 房间，然而并非 DC，对 gamma 光线敏感照耀。对老鼠 MHC-II 和 CD4 的 Monoclonal 能堵住 ATL1 增长的调停 DC 的刺激，显示在 ATL1 andAPCs 之间的血缘的识别。我们的数据建议包含巨噬细胞， B 房间和 autoreactiveT 房间的积极反馈环可以在保留导致自体免疫病的自体免疫的回答的动量起一个枢轴的作用。

Systemic lupus erythematosus （SLE） is a typical autoimmune disease involving multiple systems and organs. Ample evidence suggests that autoreactive T cells play a pivotal role in the development of this autoimmune disorder. This study was undertaken to investigate the mechanisms of interaction between antigen presenting cells （APCs） and an autoreactive T cell （ATLI） clone obtained from lupus-prone BXSB mice. ATLI cells, either before or after 7-ray irradiation, were able to activate naive B cells, as determined by B cell proliferation assays. Macrophages from BXSB mice were able to stimulate the proliferation of resting ATL 1 cells at a responder/stimulator （R/S） ratio of 1/2.5. Dendritic cells （DCs） were much more powerful stimulators for ATLI cells on a per cell basis. The T cell stimulating ability ofmacrophages and B cells, but not DCs, was sensitive to T-ray irradiation. Monoclonal antibodies against mouse MHC-Ⅱ and CD4 were able to block DC-mediated stimulation of ATL 1 proliferation, indicating cognate recognition between ATL 1 and APCs. Our data suggest that positive feedback loops involving macrophages, B cells and autoreactive T cells may play a pivotal role in keeping the momentum of autoimmune responses leading to autoimmune diseases.