保肝解毒颗粒对四环素致急性肝损伤小鼠肝细胞超微结构的立体计量学分析

Tridimensional metrological analysis of Baogan Jiedu Granule on liver cell ultrastructure of model mice with acute liver injury induced by tetracycline

目的探讨保肝解毒颗粒对四环素致急性肝损伤小鼠肝细胞超微结构的影响。方法60只小鼠随机分为模型组、保肝解毒颗粒大、中、小剂量组、甘利欣对照组和空白对照组,分别以相应剂量药物灌胃5 d,然后以四环素灌胃造模。对肝组织进行电镜观察,并对肝细胞超微结构进行立体计量学测定分析。结果模型组线粒体、异染色质Vv及线粒体、内质网、高尔基体δm明显降低,常染色质和脂滴Vv明显升高;保肝解毒颗粒可明显升高线粒体、异染色质Vv及线粒体、内质网、高尔基体δm,降低常染色质和脂滴Vv。结论保肝解毒颗粒可使肝细胞超微结构变化显著改善,具有较好的保肝作用。

Objective To explore the effect of Baogan Jiedu Granule on liver cell ultrastructure of model mice with acute liver injury induced by tetracycline. Methods Totally 60 mice were randomly divided into tetracycline model group, large-dosage, medium-dosage and small-dosage groups of Baogan Jiedu Granule, Ganlixin group and control group. Each group was respectively perfused with corresponding drug for 5 days and tetracycline was perfused once to make the model. The tissues were observed by electron microscope, and the liver cell ultrastructure was measured and analyzed by three-dimensional metrology. Results The amount of mitochondria Vv, heterochromatin Vv, mitochondria δm, endoplasmic reticulum ;m and Golgi＇s apparatus δm all obviously decreased in model group, while that of euchromatin Vv and lipid droplet Vv increased. Baogan Jiedu Granule could remarkably increase the amount of indexes referred above, and decrease the amount of euchromatin Vv and lipid droplet Vv. Conclusion Baogan Jiedu Granule can significantly ameliorate the pathologic change of liver cell ultrastructure, thus having a good hepatoprotective effect.