金纳多对抗大鼠脑出血后脑水肿的作用机制

Rivalry mechanism of ginkgo in rat brain edema after intracerebral hemorrhage

目的探讨金纳多对抗脑出血后脑水肿形成的作用机制。方法选健康Wistar大鼠80只,雌雄不拘,随机分为4组,每组20只。采用立体定向注射方法,用微量注射器在右侧尾状核部位,注入50μL生理盐水(A、B组)或等量自体血红蛋白(C、D组)。术后A、C组给予50 g/L羧甲基纤维素钠溶液3 mL灌胃,B、D组给予等量羧甲基纤维素钠溶解均匀的金纳多(200 mg/kg)溶液灌胃,每日1次,持续3周。断头取脑,测定超氧化物歧化酶(SOD)、丙二醛(MDA)值及血红素氧合酶-1(HO-1)阳性细胞数。结果除A、B组比较无明显差异外,其余各组间SOD、MDA含量及HO-1阳性细胞数比较差异均有统计学意义(P<0.05)。结论金纳多可以抑制血红蛋白所引起的脑水肿。

Objective To investigate the rivalry mechanism of ginkgo in rat brain edema after intracerebral hemorrhage. Methods Totally 80 healthy Wistar rats were divided into 4 groups randomly （20 rats in each group）. Fifty microliters saline （group A and group B） or auto-hemoglobin （group C and group D） was infused into the right caudate nuclears using stereo tactic guidance. Groups A and C were lavaged 50 g/L carboxymethyl cellulose sodium solution of 3 mL, groups B and D were lavaged the same amount of ginkgo （200 mg/kg） solution dissolved by 50 g/L carboxymethyl cellulose sodium solution once a day for 3 weeks. All the rats were decapitated after raised for 3 weeks, and superoxide dismutase, malondialdehyde, and the number of hemeoxygenase-1 （HO-1） positive cells in brains were assayed. Results The differences in SOD and MDA contents as well as HO-1 positive cell number between every two groups were significant （P〈0.05） except group A and group B. Conclusion Ginkgo can inhibit brain edema after intracerebral hemorrhage caused by hemoglobin.