Livinα在膀胱移行细胞癌组织中表达的研究

Study on the expression of Livin gene in bladder transitional cell carcinoma

目的:研究膀胱癌相关Livin基因在膀胱移行细胞癌组织中的表达以及Livin基因表达与膀胱移行癌生物学行为的关系;研究抑癌基因p53在膀胱癌组织中突变以及Livin表达与p53基因突变的关系。方法:100例膀胱移行细胞癌组织,设计Livinα引物,以半定量RT-PCR的方法,检测膀胱癌组织Livinα mRNA,以免疫组化法检测癌组织中p53蛋白。将膀胱肿瘤病理分级、临床分期、是否复发等生物学行为及p53蛋白染色强度分别量化,与组织Livinα mRNART-PCR产物量进行等级相关分析。并以10例正常膀胱黏膜作为对照。结果:100例膀胱移行细胞癌,67例检测到Livinα mRNA表达,10例正常膀胱黏膜组织未检测到Livinα mRNA表达,Livinα mRNA在膀胱移行细胞癌组织中表达率高于正常膀胱黏膜,χ2=14.4,P=0.000。Livinα mRNA在膀胱癌组织中表达与p53蛋白阳性(rs=0.465,P=0.002)及肿瘤病理分级(rs=0.463,P=0.002)有相关性。结论:膀胱移行细胞癌组织中可见Livinα mRNA高表达,Livinα mRNA表达与膀胱癌病理分级及癌组织中p53蛋白呈正相关。

OBJECTIVE： To investigate Livin gene expression in bladder transitional cell carcinoma and explore its relationship with p53 gene expression in bladder cancer and clnicopathological futures of these lesions. METHODS： Reverse transcription polymerase chain reaction （RT-PCR） and immunohistochemical staining were employed respectively to evaluate the Livin a mRNA expression and the p53 protein expression in 100 samples of bladder cancer, together with 10 samples of normal bladder tissue. RESULTS： Of the 100 bladder cancers, Livin a mRNA expression was detected in 67 cases, whereas, no Livin a mRNA expression was found in 10 normal bladder tissue. The positive rate of Livin a mRNA expression in bladder cancer was statistically higher than that in normal bladder tissue, x^2= 14.4, P= 0. 000. The Livin α mRNA expression was correlated with p53 protein expression （rs= 0. 465, P= 0. 002） and the bladder cancer pathological grade （rs= 0. 463,P=0. 002）. CONCLUSION： The data of this study suggests that Livin α mRNA expression may be an objective parameter to predict the biological behavior and prognosis of bladder cancer.