大鼠脑干前庭核5-HT2A受体mRNA表达水平在晕船适应中的变化

Changes of serotonin 2A receptor mRNA expression level in rat vestibular nuclei during the adaptation of sea-sickness

目的探讨晕船适应过程中大鼠脑干前庭核5-HT2A受体 mRNA的变化。方法将40只SD大鼠随机分为空白对照组、晕船刺激1d组、3d组、7d组和14d组,利用晕船模拟装置对大鼠进行晕船刺激,根据大鼠脑图谱对前庭核进行取材,抽提总RNA,反转录后进行实时定量聚合酶链反应检测。结果晕船刺激1d、3d、7d、14d的相对表达量分别为对照组的3.35、3.54、1.23、1.02倍。晕船刺激第1天、第3天5-HT2A受体 mRNA显著升高(P<0.01),7d回落到空白对照组水平。结论晕船刺激可以引起大鼠前庭核5-HT2A受体 mRNA升高。

Objective To investigate the changes of 5-HT2A receptor mRNA in rat vestibular nuclei during the adaptation of sea-sickness. Methods 40 SD rats were randomly distributed into five groups： control group, motion-sickness stimulation groups of 1-day, 3-day, 7-day and 14-day. Sea-sickness stimulation on rats was imitated by crampton facility, and vestibular nuclei were distracted according to the rat brain map. Real-time fluorescence quantitative PCR with SYBR Green I technique was taken to detect the changes of 5-HT2A mRNA expression level. Results 5-HT2A receptor mRNA relative expression levels of sea-sickness 1-, 3-, 7- and 14-day groups were 3.35, 3.54, 1.23 and 1.02 times higher than those of the control group respectively. The levels of 5-HT2A receptor mRNA expression of sea-sickness significantly enhanced and kept high in the first and third day, and returned to the regular levels in the seventh day, Conclusion Sea-sickness stimulation might induce the enhance of S-HT2A receptor mRNA in rat vestibular nuclei.