摘要
利用SSR标记研究了60个山西省主要玉米(Zea maysL.)自交系的遗传变异。用23对扩增带型稳定的引物,从供试自交系中检测出91个等位基因变异,每对引物检测出等位基因2-7个,平均为3.96个,平均多态性信息含量为0.600。UPGMA聚类分析将60个自交系划分为唐四平头,旅大红骨,PN,BSSS,Lancaster,Suwan等6个类群,化群结果与其系谱关系和育种家经验基本一致。
Simple sequence repeats (SSRs) markers were used to detect genetic variation of 60 elite maize inbred lines. Twenty-three SSR primers giving stable amplified detected 96 alleles among the inbred lines tested. The average number of allele per SSR locus was 3.96 with an range from 2 to 7 .The value of polymorphism information content(PIC) for each SSR locus varied from 0.15 to 0.80 with an average of 0.600.The UPGMA analysis classified 60 inbred lines into 6 clusters, which corresponed to the heterotic groups determined by their pedigree information and breeder's experiences(i, e. Tangsipingtou, Luda Red Cob, PN, BSSS, Lancaster, Suwan)
出处
《华北农学报》
CSCD
北大核心
2007年第3期25-29,共5页
Acta Agriculturae Boreali-Sinica
基金
山西省回国留学人员基金项目(2001068)
山西省自然科学基金项目(20031080)
关键词
SSR标记
玉米
自交系
Simple sequence repeats marker
Maize
Inbred lines