人体冠心病心肌组织cTnI免疫组织化学研究

Research on cTnI Immunohistochemistry of Human Myocardium in Coronary Heart Disease

目的研究cTnI免疫组织化学法诊断人体急性心肌梗死的法医病理学意义。方法应用免疫组织化学法检测人体冠心病心肌组织cTnI的变化,进行图像分析,与HE染色法进行比较。结果人体冠心病心肌组织急性心肌梗死区域心肌细胞内cTnI免疫组化染色缺染、分布失规律性;梗死修复区域纤维间质cTnI免疫组化染色弱阳性;陈旧性心肌梗死瘢痕区域纤维间质cTnI免疫组化染色阴性。失血性休克组近心内膜心肌细胞内cTnI免疫组化染色轻度增强,并见点灶状心肌细胞内cTnI免疫组化染色缺染。结论cTnI免疫组织化学检测可以应用于人体急性心肌梗死的诊断。

Objective To investigate the signification of cTnI immunohistochemistry ot human myocardium in coronary heart disease. Methods Immunohistochemistry and image analysis were used to detect the changes of cTnI in the human myocardium, and to compare the sensitivity of immunohistochemistry and HE staining. Results In the group of patients died from coronary heart disease, the acute myocardial infarcted areas showed significant reduction of cTnI immunostaining and loss of the normal cross-strlation; the granulation tissue showed weak positive staining; the fibrous scars showed negative staining. In the group of patients with hemorrhagic shock, the myocardium showed focal reduction of myocardial cells and loss of the pronounced normal cross-striation in subendocardial myocardium. Conclusion cTnI is sensitive in diagnosis of acute human myocardial infarction.