过氧化氢诱导PC12细胞损伤的模型的建立

Establishment of A PC12 Cell Injury Model Induced by H_2O_2

目的建立不同浓度的H2O2诱导PC12细胞凋亡的模型,研究组织缺血、自由基损伤的机制。方法分别以不同浓度的H2O2处理PC12细胞12h,用噻唑蓝(MTT)比色法和流式细胞术检测处理后细胞的生长活力和凋亡情况。结果以0浓度组作为对照,100、300nmol/L的H2O2处理的PC12细胞生存率分别为(76±7.69)%、(39±7.08)%(P<0.01);0、100、300nmol/L的H2O2处理的PC12细胞后凋亡率分别为2.47%、6.00%和55.54%。结论建立了以0、100、300nmol/L的H2O2诱导PC12细胞凋亡的模型。

Objective To establish an apoptosis model of PC12 cell with different concentration H2O2 and to study the mechanism of tissue ischemia and free radical injury. Methods After 12 hour treatment with different concentrations of H2O2, the viability and apoptosis of PC12 cells were determined by 3-（4,5-Dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetrazolium bromide（MTF） and flow cytomertry （FCM） methods. Results The viability of PC12 cells induced by 100 nmol/L and 300 nmol/L H2O2 was （76.00±7.69）% and （39.00±7.08）%, respectively, compared to the 0 nmol/L group （P〈0.01）. The apoptosis ratio in PC12 cells induced by 0 nmol/L, 100 nmol/L, and 300 nmol/L H2O2 were 2.47%, 6.00%, and 55.54%, respectively. Conclusion A PC 12 cell injury model induced by different concentrations of H2O2 for the study of apoptosis was successfully established.