釉基质蛋白联合骨髓基质细胞促进牙周再生

Combination of EMPs and BMSCs in promoting regeneration of periodontal tissue

目的探讨以骨髓基质细胞(BMSCs)为种子细胞,以釉基质蛋白(EMPs)为生长因子,利用组织工程技术修复牙周骨缺损的可行性。方法选取2只健康雄性恒河猴,体外分离髂骨BMSCs并传代培养,第3代细胞与Bio-oss胶原复合。动物双侧上、下后牙区,分别制备人工水平型牙周骨缺损模型,分为空白对照组、单纯材料组、细胞/材料组和细胞/材料/EMPs组,8周时取材进行组织学和Micro-CT观察分析。结果空白对照组为纤维结缔组织形成;单纯材料组可见牙槽骨新生;细胞/材料组可见牙周再生,但新生的牙骨质较少且不规则;细胞/材料/EMPs组新生牙槽骨较多,有规则且连续的牙骨质形成。结论以BMSCs为种子细胞联合EMPs诱导,能显著促进牙周组织再生。

Objective To evaluate the feasibility of reconstructing horizontal periodontal bone defects by tissue engineering based on bone marrow stromal cells（BMSCs） as seed cells and enamel matrix proteins（EMPs） as growth factors. Methods Two healthy rhesus monkeys were selected, and BMSCs were isolated from iliac marrow and serial subcultivation was conducted. The cells of induced BMSCs at passage 3 were harvested and mixed with Bio-oss collagen. The models of horizontal periodontal bone defects were established surgically in each buccal side of the posterior teeth, and were divided into four groups （blank control group, material group, cells/material group and cells/material/EMPs group）. The histological and Micro-CT observation were carried out 8 weeks later. Results In the blank control group, the defects were filled with fibrous connective tissue. There was newly-formed alveolar bone in the material group. In the cells/material group, periodontal regeneration could be observed, while the newly-formed cementum was irregular and less in quantity. In the cells/material/EMPs group, the amount of newly-formed alveolar bone was larger, and the newly-formed cementum was continuous and regular. Conclusion The tissue engineering technique of BMSCs as seed ceils in combination with EMPs induction can significantly promote the regeneration of periodontal tissue.