牙周病患者龈下菌斑中古细菌的分布

Distribution of Archaea in subgingival plaque of patients with periodontal disease

目的建立牙周袋龈下菌斑中古细菌分布的基线信息,探讨其分布与牙周病发生的关系。方法运用古细菌通用引物扩增菌斑标本,PCR产物连接TA载体,挑选多个克隆测序。其结果使用数据库比对并构建系统发育树分析。随机采集牙周病患者龈下菌斑标本,按照牙周袋深度分组(A、B、C、D组),检测并比较各组菌斑中古细菌的检出率。结果测序结果证实扩增产物为古细菌。临床标本中牙周袋较深组(C组和D组)的古细菌检出率显著高于浅牙周袋组(A组和B组)。结论古细菌的检出率随牙周袋探诊深度的增加而增高。提示古细菌可能参与牙周病的发病。

Objective To obtain the baseline information on the distribution of Archaea in the subgingival plaque of periodontal pocket and to explore the relationship between the distribution and periodontal disease. Methods Dental plaque was amplified by Archaea universal PCR primers, which were tested by sequence analysis of the TA clone of amplified products. The validity of PCR was evaluated by Blast program and phylogenetic tree. Subgingival plaques of patients with periodontal disease were randomly collected, and were divided into four groups （group A, B, C and D） according to the depth of periodontal pocket. The detection rate of Archaea in each groups were compared. Results It was proved by sequence analysis that the amplified products were Archaea. The detection rate of Archaea in group C or D was significantly higher than that in A or B. Conclusion The prevalence of Archaea is associated with the probe depth of periodontal pocket, which indicates that Archaea may participate in the pathogenesis of periodontal disease.