摘要
为优化牛体外培养系统,本研究利用微滴培养法,探讨了卵巢状态所获卵母细胞、种公牛个体、受精时间、季节及共培养对体外培养结果的影响。结果表明:(1)不同卵巢状态所获卵母细胞的分裂率、囊胚率差异均不显著(P>0.05);(2)不同种公牛精液进行体外受精,结果显示分裂率和囊胚率差异显著(P<0.05);(3)6h、8h、12h和16h受精时间比较,结果以12h效果最佳,分裂率、囊胚率与其它各组均差异显著(P<0.05)。(4)春、秋季体外培养的效果显著优于夏、冬季。(5)使用颗粒单层可显著提高囊胚率(P<0.05)。
The paper discussed the effects of oocytes derived from slaughterhouse different states of ovaries, different semen, fertilization time, season and co-culture for in vitro culture of bovine with droplet method. （1） After culture the ooeytes derived from slaughterhouse different states of ovaries, there were no significant difference in cleavage rate and blastocyte rate. （2） There were significant difference in cleavage rate and blastoeyte rate in different semen . （3） The effect on fertilization time of 12h is better. It had significant difference compare to 6h,8h and 16h.（4） Spring and autumn had better effect than summer and winter on in vitro fertilization. （5） Granular cell layer can improve the blastocyte rate obviously, but can not improve the cleavage rate.
出处
《草食家畜》
2007年第2期27-30,共4页
Grass-Feeding Livestock
基金
新疆科技攻关项目<肉奶牛胚胎产业化及高效繁育体系的建立>
项目编号:200131102
关键词
卵巢
精液
季节
颗粒单层
ovary
semen
season
granular cell layer
