纳豆激酶液体深层发酵技术的研究

Study on Liquid Fermentation of Nattokinase

本文研究了纳豆菌株ZN-4(Bacillus natto)的液体深层发酵条件及其对纳豆激酶活力的影响,结果表明:纳豆菌株ZN-4液体深层发酵的最佳培养基配方为:葡萄糖2%,大豆蛋白胨1%,Na2HPO40.6%,NaH2PO40.1%,MgSO40.05%,CaCl20.02%;最佳培养基起始pH7.0,接种量为3%,最适发酵温度为35℃。在此条件下,摇瓶、50L罐和500L罐发酵酶活最高分别达到1903U/mL、2210U/mL和1934U/mL。

The effect of liquid fermentation on Nattokinase activity produced by Bacillus natto No,ZN-4 swain was studied, Results showed that the optimal culture conditions were 2 % glucose, 1% peptone, 0,6 % Na2HPO4, 0,1% NaH2PO4, 0.05 % MgSO4, 0,02 % CaCI2, pH 7.0, the inoculums size 3 % and temperature 35 ℃. Under these conditions, the Nattokinase activities of the strain reached 1903 U/mL in shake-flask, 2210 U/mL for 50 L fermentation, and 1934 U/mL for 500L fermentation, respectively.