纤维素酶法提取天然辣椒素的研究

Extraction of Capsaicin by Cellulose Enzyme

对酶法提取天然辣椒素的方法进行了研究,通过酶解液初始pH值、酶解时间、酶解温度和酶量的四因素三水平正交实验,得出最佳酶解条件为:在酶解初始pH为5.4,酶解温度55℃,酶解时间4h,用酶量为2.5mg/g辣椒的下,天然辣椒素产量为7.65mg/g;与乙醇浸提法提取辣椒素相比,产量比乙醇浸提法提高了33%。

The enzymolysis conditions of cellulose enzyme for extraction of Capsaicinoids, including the initial pH value of lyric enzyme solution, reaction time and temperature, were optimized here by orthogonal test. The optimized conditions were that the initial pH value was 5.4, temperature was 55 ~C, reaction rime was 4 h and the quantities of enzyme was 2.5 mg per 1 g capsicum, the yield of capsaicin was 7.65 mg/g, which is improved by 33 % compared to that by traditional method.