摘要
Objective To construct the expressing vector of shRNA/mdr 1 and to study its reversed effect in vitro. Methods 64 bp oligonucleotides of pSUPER and the targeted sequence of siRNA/mdr 1 were synthesized and annealed to from duplex strand, then were cloned into pSUPER to construct pSUPER-shRNA/mdr 1 vector.
