甲苯胺蓝染色的半定量分析在嗜碱粒细胞白血病诊断和鉴别诊断中的意义

EVALUATIONOFTHESEMI QUANTITIVEANALYEISOFTOLUIDINE BLUE STAIN IN DIAGNOSIS AND DIFFERENTIAL DIAGNOSIS OF ACUTE BASOPHILIC LEUKEMIA

应用改良Ｕｎｄｒｉｔｚ法对１２９例成人急性白血病进行甲苯胺蓝染色（ＴＢＳ）及半定量分析，试提出当白血病细胞ＴＢＳ的阳性率和积分分别大于３０％和６０％时，为考虑诊断急性嗜碱粒细胞白血病（ＡＢＬ）的标准之一。１２９例中有４例修正诊断为ＡＢＬ，原诊断均为ＡＭ－Ｍ３。通过在不同细胞中ＴＢＳ阳性和阴性对照的观察，认为该法对识别ＡＢＬ，尤其在与ＡＭＬ－Ｍ３鉴别时，是一种简单可靠的方法。

The modified Undritz's method of Toluidine Blue stain(TBS) with semiquantitive analysis was performed on the cells obtained from 129 patients with acute leukemia. One of the diagnostic criteria was suggested that TBS positive rate and score was generally over 30% and 60% , respectively, in acute basophilic leukemia(ABL). Four cases (3.4%), diagnosed primarily as acute promyelocytic leukemia(APL), were corrected to ABL among 129 patients. Observation of TBS positive and negative controls in different kinds of cells indicated that the method was a simple and valuable approach to identifying ABL and differentiating ABL from APL.