两种麻醉剂对小鼠海马神经元电活动的影响

Effect of Two Anesthetics on Activity of Hippocampal Neurons in Mice

目的研究两种常用麻醉剂戊巴比妥钠和氨基甲酸乙酯对小鼠海马CA1区神经元电活动的影响,探讨在麻醉剂作用前后,小鼠海马脑区场电位和神经元放电变化的活动规律。方法运用多通道在体记录技术,分别在5只雄性C57BL小鼠双侧海马CA1区植入12组金属电极,并记录麻醉过程中海马CA1区单个神经元的放电活动以及相应脑区场电位的活动状况,并加以分析。结果(1)戊巴比妥钠和氨基甲酸乙酯麻醉作用过程中,小鼠海马CA1区脑电场电位变化有明显特征。(2)海马CA1区神经元在不同麻醉剂作用下放电频率变化具有规律性。结论小鼠海马CA1区脑电场电位在不同麻醉剂作用下有不同的变化规律,不同类型的神经元在不同麻醉剂的作用下其放电频率的变化也有所不同,提示不同麻醉剂对海马神经元的电活动有不同的作用机制。

Objective To compare the effects of Pentobarbital sodium and Urethane on neural discharge ofhippoeampal CA1 region in mice, and to investigate the changes of field potentials and neural firing rates ofhippocampal CA1 region after injected two anesthetic agents respectively. Methods Five. male C57BL mice were used in this study. 12 tetrodes were implanted separately on the both sides of hippocampal CA1 region. Local field potentials and the neural firing activities were recorded by using the multi-channel recording technology. Results It showed the changes of field potentials and neural firing rates in hippocampal CA1 region under anesthetization of Pentobarbital sodium and Urethane respectively. Conclusions There are different effects of two anesthetics on hippocampal neural discharge and field potentials, suggesting that Pentobarbital sodium and Urethane have different mechanisms underlying neural firing activities.