雄性F344大鼠自发性不育症与精子参数及精子核成熟度关系研究

Study on Semen Parameters and Maturation of Sperm Nucleus in Male Fishar344 Rats with Spontaneous Infertility

目的初步探讨大龄雄性F344大鼠自发性不育症与精子参数、生精细胞及精子核成熟度之间的关系。方法4、7、10、13月龄雄性F344、SD、Wistar大鼠分别记为A1、A2、A3、A4组,B1、B2、B3、B4组,C1、C2、C3、C4组,测定各组精子参数、生精细胞和精子核成熟度并与A4组作比较。结果A4组精子存活率除了与A3组无统计学差异(P≥0.05)外,显著低于其它组(P<0.01);其精子畸形率不仅高于C1组(P<0.05),且显著高于其它组(P<0.01);其精子活动力a级和b级均显著低于其它组(P<0.01);其精子核苯胺蓝染色++率显著高于其他组(P<0.01),阴性率显著低于其他组(P<0.01)。结论精子存活率减少、畸形率高、活动力低下、核成熟度异常可能是大龄雄性F344大鼠生殖力明显下降或自发性不育症的病因。

Objective To explore the relation between semen parameters and maturation of sperm nucleus and spontaneous infertility in male Fisher344 rats. Methods Thirteen months old male Fisher344 rats（group A4） were studied comparatively with other group rats on semen parameters and spermatogenic cells and maturation of sperm nucleus. The other group rats were 4, 7, 10 months old male Fisher344 rats（group A1, A2, A3） and 4, 7, 10, 13 months old male Sprague-Dawley rats（group B1, B2, B3, B4） and Wistar rats （group C1, C2,C3,C4）. Results The percentage of sperm viability of group A4 was lower significantly than that of the other groups （P〈0.01） except group A1 （P ≥ 0.05）.The percentage of abnormal morphological sperm of group A4 was more significantly than that of the others （P〈0.01 or P 〈0.05）.The percentage of ＂a＂ and ＂b＂ grade motility of sperm was lover significantly than that of the other groups （P〈0.01）.The percentage of Aniline blue strongly positive in sperm nucleus of group A4 was more significantly than that of the others （P〈0.01）. The percentage of Aniline blue negative in sperm nucleus of group A4 was lower significantly than that of the others （P〈0.01）. Conclusions Spontaneous infertility or decrease of fertility in male Fisher344 rats maybe due to the lower sperm viability, motility and maturation of sperm nucleus, and more percentage of abnormal morphological sperm.