IVF-20培养液用于四个品系大鼠体外受精的初步研究

Studies on In Vitro Fertilization by Using IVF-20 Media in Four Strains of Rat

目的探讨人用体外受精液用于实验大鼠体外受精的可行性,为实验大鼠的胚胎保种提供参考。方法选用人体外受精的IVF-20培养液作为大鼠精子获能和受精培养液,对SD、Wistar、GK、F344等四种不同品系的实验大鼠进行体外受精。并用mR1ECM培养液对SD大鼠体外受精所得胚胎进行体外培养试验。结果四个品系大鼠体外受精后的2细胞发育率分别可达83.2%、72.6%、87.8%和71.6%。体外受精的2细胞胚胎能够在体外进一步发育,SD大鼠囊胚发育率为16.7%,与体内受精胚胎在体外培养的囊胚发育率(24.5%)差异不显著。结论用于人体外受精的IVF-20培养液同样适合于实验大鼠精子的体外获能和受精,可以在多种品系获得较高的2细胞发育率,并且所得2细胞能够在体外发育到囊胚。

Objective To investigate the feasibility of IVF-20 medium on the in vitro fertilization （IVF） in Rats. Methods Using IVF-20 as capacitation and fertilization medium, the IVF in four swains, including SD, Wistar, GK and F344 were conducted. Besides, the embryos from SD rat fertilized both in vitro and in vivo were cultured using mR1EMC medium to test their viability. Result The fertilization rates in SD, Wistar, GK and F344 were 83.2%, 72.6%, 87.8% and 71.6% respectively. The rate of embryos developed to blastula was 16.7%, having comparative viability of embryos fertilized in vivo （24.5%）. Conclusion The IVF-20 medium, which had been used in vitro fertilization of human, is also a suitable medium for the capacitation and fertilization in rats.