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TACO与细胞膜具有非依赖肌动蛋白方式的结合 被引量：2

Binding between TACO and cell membrane is in a non-actin related way
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摘要 目的探讨TACO与细胞膜之间的相互作用。方法利用免疫荧光分析检测内源性TACO在细胞内的分布情况,然后利用细胞膜内膜表面展示分析和差速离心分离亚细胞组分分析检测TACO蛋白与细胞膜的结合,以及该结合与细胞膜上肌动蛋白纤维之间的相互关系。结果TACO主要分布在细胞膜和核膜附近区域。结论TACO与细胞膜具有非依赖肌动蛋白方式的结合,这一结果为阐释TACO在细胞吞噬初始阶段的功能提供了有力的实验依据。 Objective To analyse the interrelationship of tryptophan-aspartate-containing coat protein （TACO） and cellular membrane, and try to understand the molecular mechnism and function. Methods Immunofluorescence analysis was used to test the endogenous distribution of TACO in the cell. And then the inner membrane surface display analysis and differential centrifugation separation inferior celelular component was used to detect the binding between TACO and cellular membrane and their binding＇s relationship with the actin-binding proteins fibers. Results TACO was mainly distributed near the cellular membrane and nuclear membrane. Conclusion The binding between TACO and cell membrane is non-actin related. The result can provide evidence for exploring the function of TACO at the early stage of cell phagocytose.
作者 黄鹏 金萌萌 曲国蕃 唐佩福 王岩松 郝克强 王玉学
机构地区 中国人民解放军总医院骨科 哈尔滨医科大学第二临床医学院骨科 哈尔滨医科大学第三临床医学院骨科
出处 《哈尔滨医科大学学报》 CAS 北大核心 2007年第3期241-243,256,共4页 Journal of Harbin Medical University
关键词 TACO 细胞膜 肌动蛋白 TACO plasma membrane actin
分类号 Q73 [生物学—分子生物学]
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参考文献10

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二级参考文献46

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共引文献3

同被引文献14

  • 1刘长振,何立,沈庆涛,李湘辉,隋森芳.亮氨酸拉链结构域在p57与actin结合中的重要作用[J].生物化学与生物物理进展,2005,32(8):718-725. 被引量：4
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  • 7Grogan A, Reeves E, Keep N, et al. Cytosolic phox proteins interact with and regulate the assembly of coronin in neutrophils [ J ]. J Cell Sci, 1997, 110( Pt 24):3071-3081.
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引证文献2

哈尔滨医科大学学报
2007年 第3期

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